Regulatory

Part:BBa_K3726079:Design

Designed by: Santiago Barrag�n Ariza   Group: iGEM21_MADRID_UCM   (2021-10-08)


P_PpsbA*-RiboJ


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 234
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 234
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 234
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 234
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 234
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a promoter part, the upstream overhang is GGAG, while the downstream overhang is TACTAGAG , where AGAG are extra bases for optimal ribosomal binding, in accordance with the Marburg Collection design guidelines.

Source

PpsbA-RiboJ includes two components, PpsbA* and RiboJ. PpsbA* is an hybrid promoter where the strong light-inducible PpsbA promoter from Amaranthus hybridus and the Cu2+ inducible promoter petE from Synechocystis PCC6803 has been combined. RiboJ is an insulator (BBa_K1679038).

References

B. Wang, C. Eckert, P. Maness and J. Yu, "A Genetic Toolbox for Modulating the Expression of Heterologous Genes in the Cyanobacterium Synechocystis sp. PCC 6803", 2021.

P. Till, J. Toepel, B. Bühler, R. Mach and A. Mach-Aigner, "Regulatory systems for gene expression control in cyanobacteria", 2021.